BD Biosciences brand of digital flow cytometers, including the FACSCanto, the LSR-II, FACSAria and Fortessa, utilize a software acquisition program known as FACSDiva.
FACSDiVa Software. The software uses a database server software to manage the flow cytometry data. All data, plots, gates, instrument settings, and statistics are saved inside the database. The user cannot specify any path for saving, everything is done automatically in the background.
- BD FACSDiva software is a flexible data acquisition and analysis package specifically designed for digital-based flow cytometers. The software uses flexible features to simplify acquisition, including Experiment templates, user-definable experiment layouts, and automated compensation calculation.
- BD Biosciences welcomes customer input on corrections and suggestions for improvement. BD FACSDiva software © Becton, Dickinson and Company. This software is the property of Becton, Dickinson and Company. Each sale of a stored unit of this software grants the purchaser a nontransferable, nonexclusive, personal license.
Diva is aptly named as it can be a difficult program to master.
However, Diva has come along way over the past 10 years and many improvements have been made to help end-user.
Taking time to learn these changes will improve the reproducibility of your data, the chances of your data getting published, and your overall experience on the cytometer.
These changes will also save you time. Here are 8 time-saving FACSDiva tips to use the during your next flow experiment:
1. Compensation doesn’t require the ‘universal negative.’
It is important to remember that the background of the positive and negatives should be matched. The use of a universal negative will cause problems in compensation, especially if cells and beads are used for generating the compensation matrix.
In Diva, you can unselect the ‘use a separate tube for negative control’ when you’re setting up compensation. This will allocate the placement of a P3 gate on the single-stained histograms. Diva will then use the P3 gate as the negative population when calculating compensation.
2. Make multiple matrices.
In the case where several different antigens are being measured in the same channel, especially where the label is a tandem dye, it is critical to have a different compensation matrix for each tandem combination.
In Diva, when you create a compensation matrix, you can add additional columns of the channel in question, and label each one with the appropriate antigen name. Then, after collecting the controls and performing compensation, when you are finally collecting your samples, make sure each one is labeled with the same name. This will ensure that the proper compensation matrix is applied to each sample.
3. Collect more events.
When calculating compensation, don’t let the default of 5,000 events be your guide. When using compensation particles, 10,000-20,000 events is better. When using cells, 30,000-50,000 is better. A simple change in your DIVA layout can ensure that sufficient events are collected.
4. Create keywords.
Annotation of the data with keywords will help in finding data weeks and months after the experiments are run. Keywords are also useful when performing analysis in third party software for grouping and batch analysis. If you don’t know how to add keywords to your experiment, you are wasting valuable time that will affect your ability to reproduce and publish your data. Learn how to create keywords here.
5. Keep the Diva database clean.
Diva is based in Java, and the database size can have a negative impact on the speed of the software. It’s best to keep the data base seize well under 15 gigabytes. This means you need to export your experiments on a regular basis. It also means you need to back up your database before making major changes to your computer or the software program itself.
6. Limit the size of your data files.
Facsdiva Software Download
With the ability to measure a million events in a few minutes, and with the occasional need to look at rare events, it is important to remember the size of file should be kept to a minimum.
The best practice is to collect three or four 1-2 million event files versus, for example, one 10 million event file. The data can later be joined (concatenated) in third party software platforms. But, during acquisition, to keep processing times reasonable, don’t let the file size get to big.
7. Read the Cytometer Setup & Tracking (CS&T) baseline report.
This report, which Diva provides, is full of useful information. The report includes suggestions for starting voltages, PMT dynamic ranges, and more.
When setting up a new experiment review these settings, especially the linear dynamic range of the PMTs. If your signals are outside of this range, then any compensation you apply to your cells will not be correct.
8. Use application specific settings.
When performing longitudinal studies, it’s essential that you maintain the optimal voltage of the instrument. This will ensure that any differences discovered will be the result of the biology of your cells, not the settings of your instrument.
Application specific settings should be finalized when the experiment is started. These settings are linked to the daily CS&T reports and, if the settings are correct, the software will make the appropriate adjustments in response to any instrument changes.
Expanding confidence and flexibility in today’s integrated laboratory BD FACSDiva™ software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for today’s busy laboratory. BD FACSDiva software provides new features to help users integrate flow systems into new application areas, including index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories.
[Cytometry] diva 8 stand alone on windows 64 bit computer [Cytometry] diva 8 stand alone on windows 64 bit computer prateek arora Thu Aug 31 00:41:53 EDT 2017 • Previous message: • Next message: • Messages sorted by: Dear Dr. Rose,Thanks for sharing these steps and information to install Diva software on Windows 7 64-bits system. I am sure it will be useful for many of Diva users who are willing to install offline diva software for their data analysis.Sincerely,PrateekFrom: 'Rose, David' Sent: Wed, 30 Aug 2017 17:23:04To: ' Subject: Re: [Cytometry] diva 8 stand alone on windows 64 bit computerHi Ellen, These are the steps that I’ve used to install Diva 8.02 on Windows 7 64-bit standalone systems: Installing BD FACSDiva 8.0.2 from provided disk 1. Insert BD FACSDiva 8.0.2 disk into BR/DVD/CD drive 2. Do not allow autorun 3.
Aug 31, 2017 - Download Sentinel Protection Installer v7.6.4 Driver for Windows 7 64-bit d. Unzip and run. Open BD FACSDiva software and use for analysis as normal If you have any questions feel free to contact me. David Rose, PhD.
In Windows explorer view BD FACSDiva 8.0.2 disk files 4. Open ISSetupPrerequisites folder a. Open Java Runtime Environment folder and run JRE installer (v6u29) [don’t let Java installer install any extra software or alter internet browser settings] b. Open Sybase SQL Anywhere folder and run sqlany12 installer 5. Navigate back to BD FACSDiva 8.0.2 disk main folder 6. Run “setup” to install FACSDiva 8.0.2 following directions in software a.
Bd Facsdiva Software Reference Manual
Set up will ask what cytometer is going to be connected, select whichever cytometer you use most often. Setup will attempt to connect to cytometer but won’t be able to. Continue without installing Firmware b. Do not install HTS components 7.
Jan 16, 2017 Zemun, Veljko Kovacevic pomocnik ministra za vodni saobracaj i bezbednost plovidbe, o situaciji na z Gradska Opstina Zemun. Unsubscribe from Gradska Opstina Zemun? Created Date: Wed Aug 14 13. Zayavlenie na vidachu informacionnogo pisjma o primenenii usn obrazec d. Na temelju članka 5. Pravilnika o tlačnoj opremi (»Narodne novine«, br. 58/2010 i 140/2012), te na temelju dostavljenog popisa hrvatskih norma za tlačnu opremu od Hrvatskog zavoda za norme, ministar gospodarstva objavljuje. OBRAZEC PREDLOGA ZA VKLJUČITEV BIOSFERNEGA OBMOČJA MURA na Seznam biosfernih območij pri UNESCO Programu človek in biosfera (MAB)_št1_28092015. Pomembno funkcijo izobraževanja mladih o pomenu varstva narave in kulturne dediščine ima tudi mreža srednjih in osnovnih šol, ki deluje na območju. FILE - In this Oct. 1, 2017, file photo provided by GeoHazards Divison, Vanuatu Meteorological and GeoHazards Department, steam rises from the volcanic cone that has formed in Lake Vui near the summit of Ambae Island, Vanuatu.
Download SafeNet Sentinel (USB Dongle) driver from www.rainbow.com.my a. Once at this site click support b.
Click “Other Hardware Keys” c. Download Sentinel Protection Installer v7.6.4 Driver for Windows 7 64-bit d. Unzip and run install file for Sentinel Protection software 8. Close all programs and restart computer 9. Make sure dongle is physically connected to the computer 10. Open BD FACSDiva software and use for analysis as normal If you have any questions feel free to contact me. David Rose, PhD Staff Scientist, Analytical Cytometry Core Beckman Research Institute/City of Hope Medical Center Duarte, CA 91010 626-359-8111 x63306 ----------------------------------------------------------------------SECURITY/CONFIDENTIALITY WARNING-This message (and any attachments) are intended solely for the individual or entity to which they are addressed.
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